Androgen Receptor (06-680, Millipore, Lot number 2746312)
protein
Androgen Receptor
cell type
Metastatic, Prostate cancer cell from left supraclavicular lymph node from Lymph node
cell line
LNCaP
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin Immunoprecpitations were performed using HighCell ChIP kit following manufacturer's protocol (Diagenode). The ChIP DNA was isolated and purified (IPure Kit, Diagenode) from samples. Next- generation sequencing library preparation using KAPA Hyper Prep kit (KAPA Biosystems) using TruSeq adapters (Integrated DNA Technologies). A quality check of the purified libraries was carried out using the Bioanalyzer 2100 (Agilent).